LDHA Knockout cell line (MSTO-211H）

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LDHA Knockout cell line (MSTO-211H） Order

LDHA Knockout cell line (MSTO-211H）
Catalog#
YKO-H910
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
LDHA Knockout cell line (MSTO-211H）
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
LDHA Knockout cell line（MSTO-211H）
Cell Morphology
Epithelial-like, adherent
Passage ratio
1：3-1：4
Species
Human
Gene Name
LDHA
Gene ID
3939
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%RPMI-1640+10%FBS
Accession
CVCL_E0ZC(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
Target region
Knockout region: Exon 3 (based on transcript LDHA-205)
Validation: PCR+Sanger Sequencing
LDHA Knockout cell line (MSTO-211H） Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:MSTO-211H		 STR Info (Cell bank)
Cell Line:MSTO-211H
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 12121212
D13S317 11141114
D7S820 812812
D16S539 13131313
VWA 16181618
TH01 89.389.3
AMEL XYXY
TPOX 11111111
CSF1PO 11121112
D12S391 1819
FGA 2121
D2S1338 2024
D21S11 2831
D18S51 1618
D8S1179 1313
D3S1358 1515
D6S1043 1112
PENTAE 713
D19S433 1314
PENTAD 1112
D1S1656 16.316.3
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the LDHA gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the MSTO-211H cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, LDHA gene knockout LDHA Knockout cell line（MSTO-211H） will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
LDHA Knockout cell line (MSTO-211H）
LDHA Knockout cell line (MSTO-211H）
Catalog#
YKO-H910
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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