PFKFB3 Knockout cell line (BEAS-2B)

PFKFB3 Knockout cell line (BEAS-2B) Order

Catalog#

YKO-H1174

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

PFKFB3 Knockout cell line（BEAS-2B）

Cell Morphology

Epithelial-like, adherent

Passage ratio

1:3~1:4

Species

Human

Gene Name

PFKFB3

Gene ID

5209

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Culture method

90% DMEM+10% FBS

Target region

Knockout region: Exon 5,Exon 6,Exon 7 (based on transcript PFKFB3-203)
Validation: PCR+Sanger Sequencing

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:BEAS-2B			STR Info (Cell bank) Cell Line:BEAS-2B
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	12	13		12	13
D13S317	13	13		13	13
D7S820	10	13		10	13
D16S539	12	12		12	12
VWA	17	18		17	18
TH01	7	9.3		7	9.3
AMEL	X	Y		X	Y
TPOX	6	11		6	11
CSF1PO	9	12		9	12
D12S391	17	18
FGA	20	24
D2S1338	22	23
D21S11	28	30
D18S51	18	19
D8S1179	13	15
D3S1358	15	17
D6S1043	12	18
PENTAE	5	8
D19S433	13.2	15.2
PENTAD	3	13
D1S1656	14	14

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the PFKFB3 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the BEAS-2B cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, PFKFB3 gene knockout PFKFB3 Knockout cell line（BEAS-2B） will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

PFKFB3 Knockout cell line (BEAS-2B)

Catalog#

YKO-H1174

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

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