PIN1 Knockout cell line (Hep G2)

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PIN1 Knockout cell line (Hep G2) Order

Catalog#

YKO-HT4368

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

PIN1 Knockout cell line (Hep G2)

Species

Gene Name

PIN1

Gene ID

5300

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the PIN1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, PIN1 gene knockout PIN1 Knockout cell line (Hep G2) will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

PIN1 Knockout cell line (Hep G2)

Catalog#

YKO-HT4368

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

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PIN1 Knockout cell line (Hep G2)

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