SIK1 Knockout cell line (EA.hy926)

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SIK1 Knockout cell line (EA.hy926) Order

Catalog#

YKO-H1153

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

SIK1 Knockout cell line（EA.hy926）

Cell Morphology

Endothelial cells, adherent

Passage ratio

1:2~1:3

Species

Human

Gene Name

SIK1

Gene ID

150094

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Culture method

90%DMEM+10%FBS

Target region

Knockout region: Exon 5 (based on transcript SIK1-201)
Validation: PCR+Sanger Sequencing

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:EA.hy926			STR Info (Cell bank) Cell Line:EA.hy 926
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	11	11		11	11
D13S317	11	11		11	11
D7S820	8	9	10.0	8	9	10.0
D16S539	11	12		11	12
VWA	14	17		14	17
TH01	6	8	9.3	6	8	9.3
AMEL	X	X		X	X
TPOX	8	9		8	9
CSF1PO	10	11	12.0	10	11	12.0
D12S391	15	18
FGA	22	23
D2S1338	22	24
D21S11	28	29	32.0
D18S51	13	15	17.0
D8S1179	13	13
D3S1358	15	16
D6S1043	11	12	22.0
PENTAE	7	11	12.0
D19S433	13	14
PENTAD	9	11
D1S1656	12	17

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the SIK1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the EA.hy926 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, SIK1 gene knockout SIK1 Knockout cell line（EA.hy926） will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

SIK1 Knockout cell line (EA.hy926)

Catalog#

YKO-H1153

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

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SIK1 Knockout cell line (EA.hy926)

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