SIRT2 Knockout cell line (HCT 116)

SIRT2 Knockout cell line (HCT 116) Order

Catalog#

YKO-HS3358

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

SIRT2 Knockout cell line (HCT 116)

Cell Morphology

Epithelial-like, adherent

Passage ratio

1:2~1:4

Species

Human

Gene Name

SIRT2

Gene ID

22933

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Culture method

90%McCOY's 5A+10% FBS

Target region

Knockout region: Exon 4 (based on transcript SIRT2-201)
Validation: PCR+Sanger Sequencing

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:HCT 116			STR Info (Cell bank) Cell Line:HCT 116
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	10	11		10	11
D13S317	10	12		10	12
D7S820	11	12		11	12
D16S539	11	13		11	13
VWA	17	22		17	17
TH01	8	9		8	9
AMEL	X	X		X	Y
TPOX	8	8		8	9
CSF1PO	7	10		7	10
D12S391	17	21
FGA	18	23
D2S1338	16	16
D21S11	29	30
D18S51	16	17
D8S1179	12	14
D3S1358	12	19
D6S1043	13	13
PENTAE	13	14
D19S433	12	13
PENTAD	9	13
D1S1656	12	14

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the SIRT2 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the HCT 116 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, SIRT2 gene knockout SIRT2 Knockout cell line (HCT 116) will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

SIRT2 Knockout cell line (HCT 116)

Catalog#

YKO-HS3358

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Related Popular Products

Wild-type Cell Lines

Human Colon Carcinoma Cell line(HCT 116)
inquiry More details

Catalog#: YC-C004

Size: 1*10^6

Instruction:

Gene Knockout kit

EZ-editor™Gene Knockout kit
inquiry More details

Catalog#: MY-001

Size: -

Instruction:

EZ-cryo™ cell freezing medium

EZ-cryo™ Cell Freezing Medium
inquiry More details

Catalog#: YK-CR-50;YK-CR-100

Size: 50ml;100ml

Instruction:

KO Cell Lines

SIRT2 Knockout cell line (Hep G2)
inquiry More details

Organism: Human

Gene Name: SIRT2

Gene ID: 22933

Size: 1*10^6

Catalog#: YKO-HT5656

YKO-HT5656(Hep G2)
SIRT2 Knockout cell line (THP-1)
inquiry More details

Organism: Human

Gene Name: SIRT2

Gene ID: 22933

Size: 1*10^6

Catalog#: YKO-HT1291

YKO-HT1291(THP-1)
SIRT2 Knockout cell line (H9)
inquiry More details

Organism: Human

Gene Name: SIRT2

Gene ID: 22933

Size: 1*10^6

Catalog#: YKO-H1123

YKO-H1123(H9)

Related Popular Products

Wild-type Cell Lines

Human Colon Carcinoma Cell line(HCT 116)

Price (USD): -

Catalog#: YC-C004

Size: 1*10^6

Instruction:

inquiry More details

Gene Knockout kit

EZ-editor™Gene Knockout kit

Price (USD): 998

Catalog#: MY-001

Size: -

Instruction:

inquiry More details

EZ-cryo™ cell freezing medium

EZ-cryo™ Cell Freezing Medium

Price (USD): -;-

Catalog#: YK-CR-50;YK-CR-100

Size: 50ml;100ml

Instruction:

inquiry More details

KO Cell Lines

SIRT2 Knockout cell line (Hep G2)

Organism: Human

Gene Name: SIRT2

Gene ID: 22933

Size: 1*10^6

Catalog#: YKO-HT5656

inquiry More details
SIRT2 Knockout cell line (THP-1)

Organism: Human

Gene Name: SIRT2

Gene ID: 22933

Size: 1*10^6

Catalog#: YKO-HT1291

inquiry More details
SIRT2 Knockout cell line (H9)

Organism: Human

Gene Name: SIRT2

Gene ID: 22933

Size: 1*10^6

Catalog#: YKO-H1123

inquiry More details

SIRT2 Knockout cell line (HCT 116)

Ubigene