SPOP Knockout cell line(5637)

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SPOP Knockout cell line(5637) Order

SPOP Knockout cell line(5637)
Catalog#
YKO-H520
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
SPOP Knockout cell line(5637)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
SPOP Knockout cell line (5637)
Cell Morphology
Epithelial-like, adherent
Passage ratio
1:2-1:3
Species
Human
Gene Name
SPOP
Gene ID
8405
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%RPMI-1640+10%FBS
Accession
CVCL_D7IV(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
Target region
Knockout region: Exon 6 (based on transcript SPOP-206)
Validation: PCR+Sanger Sequencing
SPOP Knockout cell line(5637) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:5637		 STR Info (Cell bank)
Cell Line:5637
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11121112
D13S317 11111111
D7S820 10111011
D16S539 9999
VWA 18181818
TH01 7979
AMEL XXXY
TPOX 8889
CSF1PO 11111111
D12S391 2020
FGA 2222
D2S1338 2525
D21S11 3636
D18S51 1618
D8S1179 1010
D3S1358 1517
D6S1043 1620
PENTAE 1012
D19S433 1315
PENTAD 1111
D1S1656 1515
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the SPOP gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the 5637 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, SPOP gene knockout SPOP Knockout cell line (5637) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
SPOP Knockout cell line(5637)
SPOP Knockout cell line(5637)
Catalog#
YKO-H520
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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