TLN1 Knockout cell line(NB4)

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TLN1 Knockout cell line(NB4) Order

TLN1 Knockout cell line(NB4)
Catalog#
YKO-H400
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
TLN1 Knockout cell line(NB4)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
TLN1 Knockout cell line（NB4）
Cell Morphology
Monocyte, suspension
Passage ratio
1：2-1：4
Species
Human
Gene Name
TLN1
Gene ID
7094
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%RPMI-1640+10% FBS
Accession
CVCL_E0ZE(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
Target region
Knockout region: Exon 6 (based on transcript TLN1-201)
Validation: PCR+Sanger Sequencing
TLN1 Knockout cell line(NB4) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:NB4		 STR Info (Cell bank)
Cell Line:NB-4
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 13131313
D13S317 11121112
D7S820 101314.01013
D16S539 9999
VWA 16191619
TH01 79.379.3
AMEL XXXX
TPOX 811811
CSF1PO 11121112
D12S391 1922
FGA 202122.0
D2S1338 202325.0
D21S11 2833.2
D18S51 1214
D8S1179 1014
D3S1358 1517
D6S1043 1212
PENTAE 713
D19S433 1515
PENTAD 1013
D1S1656 1215
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the TLN1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the NB4 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, TLN1 gene knockout TLN1 Knockout cell line（NB4） will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
TLN1 Knockout cell line(NB4)
TLN1 Knockout cell line(NB4)
Catalog#
YKO-H400
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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