Mouse CRISPR Deletion Library - Membrane proteins†

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Mouse CRISPR Deletion Library - Membrane proteins†

Mouse CRISPR Deletion Library - Membrane proteins†

Catalog#： LIBR-M008-P002 LIBR-M008-P100 LIBR-M008-P200 LIBR-M008-P500

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Detailed Product Information Recommended Companion Products
The CRISPR knockout library targets 470 mouse membrane protein genes with a total of 5,550 knockout plasmid vectors, of which 10 different gRNA vectors are designed for each gene, in addition to 1,500 control vectors targeting intergenic sequences. The library uses pMCB320 as the backbone, which is a two-plasmid system that expresses only gRNA, while the Cas9 gene is on a separate vector that needs to be used in conjunction.
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Product Information

Product Name
Mouse CRISPR Deletion Library - Membrane proteins†
Organism
Mouse
Library Type
Knockout Library
Plasmid System
Dual-plasmid System
Virus Packaging System
3rd Lentivirus Packaging System
Targeted Genes
470
gRNA Number
5550
Non-targeting gRNA Number
1500
Label
Puro, mCherry
Vector Formula
pMCB320 vector formula
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CRISPR iScreen™ Product Strength
  • 35+ Libraries
    100+ Cas9 cell lines for screening
    35+ Library types in stock, fulfilling different research needs Cas9 cells with high activity, good cell condition, easily accelerate CRISPR library construction.
    1
  • Plasmid
    Coverage>99%, uniformity<10
    The use of self-developed library specific competent cell makes it easier to capture exogenous DNA, with high transformation efficiency and low mutation risk.
    2
  • Cell Pool
    Coverage rate up to 99%
    Exclusive cell pool preparation process can achieve large-scale and standardized production of library cell pool, achieving fewer differences between batches and high repeatability.
    3
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CRISPR Library Screen Service
Provides one-stop solutions for CRISPR-KO, CRISPRa, CRISPRi library screen from high-throughput sgRNA library construction, virus packaging, cell infection, drug screening, NGS sequencing to data analysis, etc. Various deliverables fulfill different research needs.
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Cas9 Stable Cell Line
The Cas9 cell lines in our cell bank can stably express Cas9 protein. So gene knockout can be achieved by transfecting gRNA. Simultaneously transfecting gRNA and donor DNA can achieve gene knock-in/point mutation, effectively improving experimental efficiency.
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