HaCaT-EGFP cell line

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HaCaT-EGFP Order

Catalog#

YC-A018-EGFP-P

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Datasheet

Order

Detailed Product Information

Product category

EGFP Stable Cell Lines

cell name

HaCaT-EGFP

Cell Morphology

Epithelial-like, adherent

Passage ratio

1：3~1：5

Species

Human

Reporter gene

EGFP

Resistance gene

puro

Construction method

Lentivirus

Mycoplasma test

negative

Culture method

90%DMEM+10%FBS

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:HaCaT			STR Info (Cell bank) Cell Line:HACAT
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	12	12		12	12
D13S317	10	12		10	12
D7S820	9	11		9	11
D16S539	9	12		9	12
VWA	16	17		16	17
TH01	9.3	9.3		9.3	9.3
AMEL	X	X		X	X
TPOX	11	12		11	12
CSF1PO	9	11		9	11
D12S391	18	23
FGA	24	24
D2S1338	17	25
D21S11	30.2	30.2
D18S51	12	12
D8S1179	14	14
D3S1358	16	16
D6S1043	12	19
PENTAE	7	12
D19S433	13	14
PENTAD	13	13
D1S1656	11	12

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use EGFP gene expressing lentivirus constructed by Ubigene to infect the HaCaT cell line， screen the cells based on the puromycin resistance marker and the optimal antibiotic screening concentration explored in advance, resulting in a HaCaT-EGFP cell line that can maintain long-term, stable and high expression.

HaCaT-EGFP

Catalog#

YC-A018-EGFP-P

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

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HaCaT-EGFP cell line

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