EPDC1 Knockout cell line (HCT116)

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EPDC1 Knockout cell line (HCT116) Order

EPDC1 Knockout cell line (HCT116)
Catalog#
YKO-H1011
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
EPDC1 Knockout cell line (HCT116)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
EPDC1 Knockout cell line (HCT116)
Species
Human
Gene Name
EPDC1
Gene ID
55635
Gene Information
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Target region
Knockout region: Exon 1 (based on transcript DEPDC1-202)
Validation: PCR+Sanger Sequencing
EPDC1 Knockout cell line (HCT116) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the EPDC1 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the HCT116 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, EPDC1 gene knockout EPDC1 Knockout cell line (HCT116) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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